Reporter

Part:BBa_K634014:Design

Designed by: Eric Walters   Group: iGEM11_Wisconsin-Madison   (2011-09-27)


Reversed J23100-alkS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 731
    Illegal NgoMIV site found at 767
    Illegal AgeI site found at 2046
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

alkS is preceded by an efficient RBS This part is reversed, as it was part of a bi-directional system.


Source

alkS came from the registry (part BBa_K398300) J23100 is a BioBrick

References